Quality control report template for absorbance, particle size and concentration of Titanium Dioxide via SpectroWorks™

This Colab-notebook plots the spectra from SpectroWorks™ and calculates the absorbance in a given range. It also calculates the particle and concentration of samples of Titanium Dioxide.

You can find the Colab-notebook here in English.

Titanium dioxide is has a variety of uses in both industry and research. It is a white, insoluble solid and is particularly useful as a pigment in both paints, food colourings, cosmetics and sunscreen. Its use as a pigment is largely due to its brightness and high refractive index, it is used in two-thirds of all pigments worldwide.
For use in sunscreens, titanium dioxide nanoparticles are used. They are not absorbed into your skin and the bright surface reflects incoming sun, allowing for protection against UVB and UVA rays. With sufficiently small particle sizes, there is minimal white appearance on the skin. However, if the size of the particles becomes too small, the effectiveness against UVA rays is decreased.
This easy to use interactive Colab notebook allows for the quick analysis of important characteristics of titanium dioxide: its absorbance, concentration, and particle size.
The following is an example of the usage of the notebook using a sample of titanium dioxide.

Test A: Absorbance of TiO2


Figure 1: The spectra for example sample of Titanium Dioxide as calculated using the NanoCuvette S™ and the SpectroWorks™ software.
The minimum wavelength, maximum wavelength and the minimum acceptable absorption are entered into the Colab.
Minimum Wavelength (nm)  210
Maximum Wavelength (nm)  290
Minimum Absorption  18


The absorption is calculated and a Pass / Fail result is produced.

Calculated Absorption  20.4953

Test B: Particle Size of TiO2 



Figure 2: A graph showing the distribution of particle number and particle diameter as calculated using the NanoCuvette S™ and the SpectroWorks™ software.


The minimum particle number, diameter and maximum variance of the particle diameter are entered into the Colab.

Particle Number (order of magnitude)  15
Diameter (nm)  50
Variance  of diameter (nm)  ± 20


The diameter and corresponding number of particles are calculated and a Pass / Fail result is produced.

Diameter of TiO2 (nm) Number of particles  Pass / Fail
51.9828 2.7178e17 PASS

52.7074 2.6898e17

Test C: Concentration of TiO2

 The known values of volume, grams, concentration, density and dilution factor are entered into the Colab. The output gives the Pass / Fail result of the total particle concentration and the target particle concentration (i.e. the particles within the diameter range specified in Test B).

Total Volume (cm3) 1000
Grams TiO2 (g) 5
Volume concentration (%) 0.00009
Density (g/cm3) 3.78
Dilution factor 20
Minimum acceptable concentrations   Pass / Fail
Tolerance for total concentration* (%)  70 PASS
Tolerance for target particle concentration** (%)  50 FAIL
* Ex. if the measured concentration must be at least 80% of the actual concentration, enter 80.
** Ex. if the measured target particle concentration must be at least 50% of the total concentration, enter 50.
SpectroWorks™ for UV-Vis spectra and NanoCuvette™ analysis comes with API access. Whether you're a student, a scientist or a technician, Colab notebooks and SpectroWorks™ can make your work around UV-Vis spectrophotometers easier and faster. 

Contact us for more information 

Copenhagen Nanosystems ApS (cphnano),
Hørmarken 2, DK-3520 Farum, Denmark
Tel: +45 36 99 27 46