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UV-Vis Knowledge Base

NanoCuvette™ One

NanoCuvette™ One

NanoCuvette ™ One enables UV-Vis spectrophotometers to measure protein and enzyme concentrations and kinetics via refractive index down to 0.5 µL.

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Get started with NanoCuvette™ One

NanoCuvette™ One brand page

How to calculate the refractive index using NanoCuvette™ One?

Where to buy NanoCuvette™ One?

Basics

Are you having problems with NanoCuvette™ One? Report your problem here and get technical support

NanoCuvette™ One - Product Brief

NanoCuvette™ One - Flyer

NanoCuvette™ One - Product Description

NanoCuvette™ One - Cleaning Manual

NanoCuvette™ One - Data sheet

NanoCuvette™ One - Made in Denmark

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Application notes

Droplets and micro-volume sample applications for NanoCuvette™ One

Sugar and Refractometry

Saliva and starch degradation

Protein concentrations and Refractometry

Honey and Water Content

Glycerol concentration and QC

Enzymes and kinetics

Coffee and Sugar

Buffers and QC

Apple juice and QC

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Frequently Asked Questions (FAQ)

Will the sample size of the volume affect the concentration when using Nanocuvette™ One?

Which UV-Vis spectrophotometers are supported with NanoCuvette™ One?

Which sample volumes can be used to make measurements with Nanocuvette™ One?

What types of samples can be measured with Nanocuvette™ One?

What path lengths are used to make measurements and is the user required to make any calculations relevant to the path length when measuring with Nanocuvette™ One?

What is an appropriate reference solution when using Nanocuvette™ One?

What do you measure with Nanocuvette™ One?

What do I need in my lab to get started with Nanocuvette™ One?

What can I use the refractive index for?

Is it possible to do kinetics with Nanocuvette™ One?

How is refractive index different from absorbance which is conventionally measured with a spectrophotometer?

Can you measure proteins with Nanocuvette™ One?

How to do 0.5 uL measurements with NanoCuvette™ One

How to do 3 mL measurements for NanoCuvette™ One

What is low volume use for NanoCuvette™ One?

What is full volume use for NanoCuvette™ One?

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Troubleshooting

Troubleshooting NanoCuvette™ One

Teaching material

Unknown honey samples- teacher

Unknown honey samples - student

Dilution series with honey - teacher

Different honeys - teacher

Different honeys - student

Dilution series with honey- student

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Solutions

Sugar/Beverages

Proteins

Enzyme/Kinetics

Clear Liquids

Webinars

Webinar about NanoCuvette™ one

White papers

White paper: Protein quantification using spectrophotometer, NanoCuvette™ One and SpectroWorks™ compared to micro-volume system

Peer-reviewed articles

Vannahme, C., Sørensen, K. T., Gade, C., Dufva, M., & Kristensen, A. (2015). Refractometric monitoring of dissolution and fluid flow. Optics Express, 23(5), 6562-6568

Vannahme, C., Dufva, M. & Kristensen, A. High frame rate multi-resonance imaging refractometry with distributed feedback dye laser sensor. Light Sci Appl 4, e269 (2015). DOI:10.1038/10.1038/lsa.2015.4

Tølbøl Sørensen, K.; Kristensen, A. Label-Free Monitoring of Diffusion in Microfluidics. Micromachines 2017, 8, 329. DOI: 10.3390/mi8110329

P.G. Hermannsson, C. Vannahme, C.L.C. Smith, K.T. Sørensen, A. Kristensen. Refractive index dispersion sensing using an array of photonic crystal resonant reflectors. Applied Physics Letters 107 (6)

P. Hermannsson, K. Sørensen, C. Vannahme, C. Smith, J. Klein, M. Russew, G. Grützner, and A. Kristensen, "All-polymer photonic crystal slab sensor," Opt. Express 23, 16529-16539 (2015).

P. G. Hermannsson, C. Vannahme, C. L. C. Smith, & A. Kristensen. Absolute analytical prediction of photonic crystal guided mode resonance wavelengths. Appl. Phys. Lett. 105, 071103 (2014)

P. G. Hermannsson, C. Vannahme, C. L. C. Smith & A. Kristensen. Accurate wavelength prediction of photonic crystal resonant reflection and applications in refractive index measurement. Sensors IEEE

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