Get started with NanoCuvette™ One

New to NanoCuvette™ One? Here is a quick guide for accurate quantification of proteins, enzymes and carbohydrates by upgrading your UV-Vis spectrophotometer to replace micro-volume instrumentation, such as Nanodrop or mySPEC systems.

The NanoCuvette™️™ One is an excellent and affordable improvement for laboratory workflows that instantly upgrades any cuvette-based UV-Vis spectrophotometer to enable quantification of kinetics and micro-volume samples replacing dedicated micro-volume instruments such as Nanodrop or mySPEC systems.BLEN_20210812_nanocuvette_one

With NanoCuvette™️™ One, proteins, enzymes and carbohydrates can be quantified in seconds with enhanced sensitivity, reliability, and reproducibility.

Embedded in the surface is a patented optical filter with anti-stiction coating that bends the light beam such that it measures compounds. Together with the user-friendly software SpectroWorks™™️ this makes it possible to calculate concentrations and other results relevant to your experiments. 



Video: How to do 0.5 uL samples with NanoCuvette™ One.

How to start using NanoCuvette™ One in five easy steps

  1. Acquire a NanoCuvette™ One box via one of our distributors (single piece units are also shipped with VWR Collection line instruments). See full list of distributors and countries here.
  2. Turn on your cuvette-based UV-Vis spectrophotometer, perform initialization and wait for the instrument to warm up for about 30 min. An overview of +400 supported UV-Vis spectrophotometers can be found here.
  3. Go to This will open SpectroWorks™ in your browser.
  4. On the SpectroWorks login screen, click on "Sign up" next to "Need an account?" and fill out your profile information. A confirmation e-mail will be sent to the provided e-mail address to validate your user profile.
  5. Log into SpectroWorks™ with your user profile. Create a new project, select the NanoCuvette™ One workflow, type in the box code and follow the instructions on the screen. Cuvette measurements and data processing can be done in less than a minute.

More information can be found in the user guide or Step-by-step video guide for SpectroWorks™.